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Early activated hepatic stellate cell-derived paracrine molecules modulate acute liver injury and regeneration

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Cells untreated or treated with 20 µM curcumin for 24 h were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates presented as kits–Vybrant™ FAM Caspase-3 and -7 Assay Kit - CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit and the Vybrant FAM caspase-8 assay kit (V35119), all from Molecular Probes, and analyzed by flow cytometry