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A study from the Netherlands set reference intervals at 100–400 nmol/l for adults age 18–64 and 120–460 nmol/l for adults ≥65 (Abildgaard, 2021)

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Tissue injury was evaluated microscopically by a blinded examiner using an Olympus BX51 microscope and Olympus 71 digital camera, saving images as uncompressed 24-bit RGB TIFF files