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The following primers were used: GAPDH: 5'-GAGGGGCCATCCACAGTCTT-3' (forward) and 5'-TTCATTGACCTCAACTACAT-3' (reverse), GHR: 5'-GATGTTCTGAAGGGATGG-3' (forward) and 5'-GTGGGACTGATGTTGACC-3' (reverse) and PCNA: 5'-GAAGCACCAAATCAAGAG-3' (forward) and 5'-CATCTCCAATATGGCTAA-3' (reverse)

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In addition, GnRH agonist was also found to have a direct antiproliferative effect on the fibroid ( GnRH antagonist is the newer member in the class of GnRH analog following GnRH agonist