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Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP

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This characteristic curve shown in Figure S1B indicates that TPE-DCV is a typical AIE active fluorogen with an f w threshold of 68%, above which the dye molecules aggregate and the emission is turned on