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Another noninvasive technique using ultrasound to measure flow-mediated vasodilation was used in two additional studies conducted by Xiang et al

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Isotope-labelled PGRMC1 proteins for NMR analyses were prepared by growing cells (BL21 (DE3)) in minimal M9 media in H 2 O or 99.9% 2 H 2 O, including ampicillin, metals, vitamins, 15 N-ammonium chloride and 13 C or 12 C glucose as sources of nitrogen and carbon, respectively